Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures

Optimization of callus induction and cell suspension cultures of Catharanthus roseus

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

Optimization of callus induction and cell suspension cultures of Catharanthus roseus

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

Well-differentiated human airway epithelial cell cultures.

Human epithelial cell cultures from superficial limbal explants

PURPOSE To study the kinetics of growth and the phenotype of cells cultured from human limbal explants in a cholera toxin-free medium with no feeder cell layer. METHODS Human organ-cultured corneas were used to prepare limbal explants (full-thickness and superficial limbal explants) and corneal stromal explants. Cell growth kinetics and phenotypes were assessed by cultivating explants in chol...

Turbulent dynamics of epithelial cell cultures

In vitro epithelial monolayers exhibit a rich repertoire of collective dynamical behaviours according to microenvironment mechanical features. We investigate the statistical and dynamical properties of human bronchial epithelial cell (HBEC) flows in bulk, by analysing their large length-scale and long time-scale spatiotemporal dynamics. Activity-driven spontaneous collective flows consist of an...